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Serial diluted GIPR-Ab was incubated with 30,000 cells in assay buffer (0.1% BSA, 500 mM 3-isobutyl-1-methylxanthine in F12 media) for 30 min at 37 C before treatment with GIP at a final concentration of 0.05 nM

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These documents support regulatory compliance requirements while enabling research facilities to implement appropriate retest or expiration protocols based on actual storage conditions experienced

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In a rat moderate contusive SCI model, subcutaneous administration of exenatide immediately post-injury and again at 7 days significantly improved hindlimb motor function without inducing hypoglycemia [66]