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Transcriptomic analysis RAW 264.7 macrophages were seeded in 6-well plates (8 10 5 cells per well) in 4 ml complete MEM and incubated at 37 C and 5% CO 2 for 16 h

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All participants were instructed to follow a diet with a 300 kcal deficit and to increase their physical activity gradually to 30-60 minutes of exercise per day

In 2015, James returned to Georgia to continue his passion for cosmetic medicine, working in plastic surgery and aesthetics