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10.1038/nature05292 Nature 141 LiuH.ZangC.YuanF.JuC.ShangM.NingJ.et al (2022)

DAF-16/FoxO directly regulates an atypical AMP-activated protein kinase gamma isoform to mediate the effects of insulin/IGF-1 signaling on aging in Caenorhabditis elegans

NOS expression assessment by qPCR Tissue samples were homogenized using the TissueLyser system (Qiagen) to ensure efficient cell disruption and RNA extraction