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It has its own formula and schedule, depending on where you are

[DOI] [PMC free article] [PubMed] [Google Scholar] 142.Hsieh T.-S., Wang W.-H., Lin S.-J

People with hyperpigmentation typically have a high concentration of eumelanin in specific areas

Always use low-binding microcentrifuge tubes and pipette tips (siliconized or polypropylene) when handling dilute solutions below 10 mcg/mL, as surface adsorption can reduce effective concentrations by 20-50% in standard labware