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CRISPR guide RNAs were designed using CHIRPOR.org 42 and were used as two-part synthetic crRNA and tracrRNA (Alt-RTM CRISPR guide RNA, Integrated DNA Technologies, Inc)

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The proportions of M1M6 and [ 3 H]BPC157 in plasma radioactive components at different sampling times demonstrated that [ 3 H]BPC157 was the main plasma component at 0.05 h (3 min) after administration and was subsequently degraded into small molecular peptide fragments in the following 0.167 h (10 min) (Figure 5A)