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The dilutions of each primary antibody were 1:2000 for iNOS (Transduction Laboratories, Becton Dickinson Biosciences, Madrid, Spain) and 1:1000 for COX-2 (Cayman Chemical Company, Montigny le Bretonneux, France), and incubated overnight at 4C followed by peroxidase-conjugated anti-rabbit IgG antibody (1:3000) for 1 h

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