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In vitro studies have utilized human umbilical vein endothelial cells (HUVECs) to investigate angiogenic activity, human fibroblast cultures for wound migration assays, and tendon fibroblast primary cultures to examine collagen synthesis rates

Tap water and boiled water are not sterile and contain pyrogens that can cause serious adverse reactions if injected

10.1016/j.ejphar.2021.174377 123 LiuJ.ZhangX.ChengY.CaoX

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1) Biomarker challenges: Classical ferroptosis markers (MDA, 4-HNE, and C11-BODIPY) are non-specific, as they are also generated by other oxidative stress pathways (e.g., ROS from mitochondrial dysfunction)