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The mice were tested in randomized orders during the experiments

CROTONOBETAINE IS A PROATHEROGENIC GUT MICROBIOTA METABOLITE OF L -CARNITINE

Antibodies against GAPDH (sc-47724), OCT4 (sc-5279), SOX2 (sc-365964), KLF4 (sc-166238), SOX17 (sc-130295), NANOG (sc-293121), SSEA-1 (sc-21702), TRA-160 (sc-21705), TRA-180 (sc-21706), FOXA2 (sc-271103), CXCR4 (sc-53534), HNF4A (sc-374229), A1AT (sc-166018), ALBUMIN (sc-271605) and AFP (sc-80464) were from Santa Cruz Biotechnology, Inc (Santa Cruz, CA)

A pulse wave velocity test can help assess arterial stiffness before high blood pressure develops