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Site-directed mutagenesis Site-directed mutagenesis of the GST clone was performed using a QuikChange mutagenesis kit (Stratagene) according to the manufacturers protocol

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Overconsumption of Omega-6 polyunsaturated fatty acids (PUFAs) versus deficiency of Omega-3 PUFAs in modern-day diets: the disturbing factor for their balanced antagonistic metabolic functions in the human body