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The first stage is Trem2-independent and involves a reduction in homeostatic microglia checkpoint genes such as Cx3cr1, P2ry12 , and P2ry13 , along with upregulation of genes including the Trem2-signaling adaptor Tyrobp, Apoe , and B2m

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For example, by integrating data from the Traditional Chinese Medicine Systems Pharmacology Database (TCMSP) and gene databases such as GeneCards, researchers can extract compound structures, target proteins, and depression-associated genes