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Like GHRS-R1a, the G protein-coupled GLP-1 receptor (GLP-1R) is also expressed in various regions of the CNS, including the hypothalamus, thalamus, brainstem, and cortical regions such as the insula, opercular cortex, frontal pole, and paracingulate gyrus (Drucker, 2022

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Following immunoprecipitation, the bead-bound SNAP/FLAG-hGLP-1R was incubated with a click chemistry mix (20 M rhodamine-azide, 1 mM TCEP, 100 M TBTA, 1 mM CuSO 4 ) for 1 hour at room temperature gently agitating to fluorescently label the PhotoClick cholesterol