The evaluation of the frequency of entries into the novel arm, the total entries into all arms, and the time spent in the novel arm serves as an indicator of memory function ( 2.7 Novel-Object Recognition Test The test, formulated by Ennaceur and Delacour, evaluates the duration animals require to exhibit a preference for exploring unfamiliar objects over familiar ones ( 2.8 Elevated plus maze test The EPM test was implemented to investigate anxiety-like behavior in rodents, following the protocol delineated by Handley and Mithani ( 2.9 Collection of the brain tissue samples Rats were euthanized through CO 2 asphyxiation within a glass chamber ( 2.10 Enzyme-linked Immunosorbent assays Brain tissues were harvested from all animals and subsequently homogenized using a neuronal lysis buffer
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2,13 Measures of insulin resistance include the Homeostasis Model Assessment (normal value 0.35)
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