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Three-dimensional (3-D) model of vascular smooth muscle cells Rat vascular smooth muscle cells were maintained in high glucose DMEM supplemented with 10% fetal bovine serum

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2500 mcg per mL / 100 units = 25 mcg/unit

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In all cases you should follow the manufacturers directions as one medical reference notes, the volume of the preparation to be used for diluting or dissolving any drug for injection is dependent on the vehicle concentration, dose and route as recommended by the manufacturer