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10.1007/s11306-016-1094-6 16 BokulichN

2.3.4 Acrosome reaction (AR) The hyperactivated spermatozoon tends to penetrate over the cumulus-oocyte-complex and attach to the zona pellucida of the egg, whereas acrosome reaction (AR) is a well-regulated exocytotic reaction in response to coordinated stimuli [90]

The debenzylated product was precipitated in hexane, dried using a centrifuge and vacuum, dissolved in basic water, and purified by dialysis with a dialysis membrane (molecular weight cut-off 3.5 kDa) against deionized water (DIW) for three days before the lyophilization for removing water and collecting the final product

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The copper complex in GHK-Cu is relatively stable, but there's no reason to stress it