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Each sample was resuspended in 20 l of LC/MS grade water and 10 l per sample was injected into a 5500 QTRAP, a hybrid triple quadrupole/linear ion trap mass spectrometer, using a quantitative polar metabolomics profiling platform with selected reaction monitoring (SRM) that covers all major metabolic pathways

Each of these cascades contributes something different

The reduction reaction was catalysed by PDI at room temperature, and the resulting aggregation of reduced insulin B chains was measured at 620 nm

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Despite the extensive and multidisciplinary research during recent years, and the growing evidence on the anatomical and physiological lines of mucosal defense, the functional aspects of protection and healing still need to be better understood