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Reverse Transcription-Quantitative Polymerase Chain Reaction The total RNA content was isolated from cells or the tumor using TRIzol reagent (Invitrogen), treated with DNase I (Thermo Fisher Scientific, Cat# EN0521), and then reverse transcribed into cDNA using a Maximal First Strand cDNA Synthesis Kit (Thermo Fisher Scientific, Cat# K1622)

The presence of 2,3-BPG in the RBC explains the observation that the O 2 affinity of purified HbA is greater than that of whole RBCs

doi: 10.3390/ijms21145168

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Without this coupling the cell cannot pull itself forward