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Such high GSSG concentrations are unlikely to occur in most subcellular compartments but could be reached in the secretory pathway or during titration experiments of transgenic cell lines with an upregulated GSSG transporter 35,63,64,77

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Ingredient Quantity Cyanocobalamin (Vitamin B12) 1000 mcg/mL Sodium Chloride 0.22% w/v Ammonium Sulfate 0.1% w/v Citric Acid 0.01% w/v Sodium Citrate 0.008% w/v Benzyl Alcohol (Preservative) 1.0% v/v

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In U87 cells, the use of metformin alone resulted in the most noticeable increase in SREBF2 (Fig