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Purified fusion GST X protein, purified Y protein, and glutathione GSH magnetic beads that can specifically bind to GST are mixed together and incubated for a certain period of time

The keloid tissues for organ culture were firstly incubated in a DMEM containing 10% FBS at 37 C for 24 h, and the medium was replaced by the DMEM containing 2% FBS, the final concentration of 25 M FOXO4-DRI was added in the medium immediately

Synthesis and Quality Assurance: PAL GHK (Palmitoyl Tripeptide-1) is meticulously synthesised through a controlled process that guarantees high purity (98%) and stability, making it suitable for precise scientific studies

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LC-MS Identity Confirmation for Each Peptide Two target masses get verified by LC-MS on every lot: 1419.55 g/mol for BPC-157 and 4963.44 g/mol for TB-500