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Proteins were transferred to a Hybond-P membrane (GE healthcare, Piscataway, NJ), blocked with 5% non-fat milk and incubated with the following primary antibodies: PPAR, C/EBP (Abcam, USA) and GAPDH (Santa Cruz Biotechnology Inc., USA), overnight at 4 C

But too much or too little fiber can lead to constipation or diarrhea