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Their findings showed elevated serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), and hepatic IL-10 expression, which increased consistently with time and altitude 37

In sheep, the central administration of leptin markedly enhances postprandial thermogenesis in both muscle and diffuse adipose depots (retroperitoneal and gluteal fat)

Aminoguanidine and N-acetylcysteine were used as standard glycation/oxidation inhibitors

Sections were dewaxed and stained with a rabbit polyclonal anti-FITC (711900, Thermo Fisher Scientific, 1/100) plus secondary Alexa Fluor 488 antibody (Life Technologies, 1/200), and guinea pig anti-human insulin (A0564, Dako, 1/1000) plus secondary Alexa Fluor 546 antibody (Life Technologies, 1/1000) prior to confocal imaging as above

Cagossi M, Salgarello M, Patrignani P, Salgarello G