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Resources and biological activities of natural polyphenols

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The resulting 2.5% SSA plasma supernatant was collected into 1.5 mL microcentrifuge tubes, flash frozen on dry ice, and stored at 80 o C for future analysis

Revealingly, one of the first attempts to get an idea on the midpoint potential of the GSH/GSSG couple back in 1952 made use of enzymatic catalysis (Rall and Lehninger, 1952): The NADPH/NADP did simply not react with the GSH system until a then newly discovered enzyme, glutathione reductase, was added to the reaction mixture

doi: 10.1038/cdd.2015.117