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The cells were then washed three times with PBS containing 1% BSA (w/v) followed by 1 h incubation with 1:1,000 anti-mouse Alexa Fluor 488 conjugated secondary antibody (diluted with PBS containing 5% BSA, Invitrogen) at RT in the dark

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Se determin el porcentaje de fragmentacin mediante la evaluacin de 500 espermatozoides de cada muestra, con microscopa ptica a 1000

Stay hydrated: Drinking plenty of water throughout the day can help with side effects like nausea and constipation

Selenium is necessary for the production of healthy sperm in fathers and for regulating the menstrual cycle in mothers