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04 Persistent injection-site reaction Rotating sites within and between abdomen quadrants, with a fresh syringe and swab per session, is the commonly reported approach

doi: 10.2337/diabetes.53.8.2079 202 SunY.ChenH.DaiJ.WanZ.XiongP.XuY.et al

Oct 2021;38(10):2519-2541

Reverse transcription-polymerase chain reaction (RT-PCR) Mouse CD8 + T cells were collected and homogenized for RNA extraction following the manufacturers instructions: total RNA of 1 10 6 CD8 + T cells was extracted with TRIzol and the concentration was detected, and TransScript One-Step gDNA Removal and cDNA Synthesis SuperMix (Beijing Quanshi Gold Bio