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Its primary advantage over sterile water for injection is its multi-use capability the benzyl alcohol preservative allows the same vial to be accessed multiple times with a sterile needle without compromising the sterility of the remaining solution, which is essential in research settings where a single stock solution vial may be used across multiple experiments or time points
This combination is typically administered in cycles10-20 days of daily injections followed by rest periods of several weeks to months
Coverslips were mounted with ProLong Gold anti-fade reagent (Invitrogen