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The role of P53 up-regulated modulator of apoptosis (PUMA) in ovarian development, cardiovascular and neurodegenerative diseases

To determine if APAP ingestion resulted in increased ROS in the Drosophila fat body, we stained APAP-treated and control early third-instar larvae with HydroCy3, a ROS sensitive dye that fluoresces in the presence of superoxide radicals 29