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Some groups are more likely to come up short, including older adults, people who eat little or no animal food, and those with digestive conditions that make absorption harder

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In addition, HILIC is usually run with MS-compatible solvents and buffers, which can provide a solution when IP-RPLC conditions cause ionic suppression , further improving sensitivity during LC-MS analysis of low-level impurities

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Results Non-targeted metabolomic analysis of rumen fluid identified -linolenic acid as a key metabolite promoting fat accumulation