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Step 3: Introduce the Diluent SLOWLY This is the moment where most mistakes happen

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Gamma/delta T cells are rare(8), although their proportion in intrathyroidal lymphocytes is higher than that in peripheral lymphocytes(9)

Hepatoblasts were incubated in hepatic maturation media at 37 C, 5% CO 2 for 14 days with media changes every 48 h ( For the growth factor HLC maturation, hepatoblasts were cultured for 14 days in hepatoblast specification medium supplemented with 10 7 M dexamethasone (Sigma Aldrich)