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After incubation, cells were washed with PBS twice, re-suspended, and immediately submitted to flow cytometric analysis using a BD Accuri C6 Plus flow cytometer (Becton Dickinson, Mountain View, CA, USA)

This dysregulation severely impairs sperm motility and acrosome reaction capacity-critical processes for successful fertilization ( 3.1.2.2 G-protein coupled receptor pathway suppression EEDs systematically suppress GPCR-mediated signaling cascades essential for steroidogenesis and germ cell proliferation

Jerlhag et al., 2009)

The remaining 16 letters were issued to various products in clinical trials