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Benefits are most evident in metabolic dysfunction, where they reduce physiological stress and complement lifestyle measures without directly modifying primary aging mechanisms

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However, some studies suggest that thigh injections may result in slightly fewer gastrointestinal side effects for some individuals compared to abdominal injections

To examine the consistency of differential expressions across cell subtypes in the v3 kit-based dataset, for each pair of cell subtypes, the union of their significant DEGs in a given comparison (i.e., age-stratified or treatment-stratified) were included