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However, in hemorrhagic post-stroke epilepsy and PTE, large amounts of Fe 2+ from red blood cells and hemoglobin are released into the brain, and these Fe 2+ ions may be involved in inducing ferroptosis ( FIGURE 2 Regulation of Ferroptosis by Cysteine-Glutathione (Cys-GSH) Redox Axis Previous studies have shown that oxidative stress causes ferroptosis (Xiao and Meierhofer, 2019)

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Similarly, polydatin, an inhibitor of glucose-6-phosphate dehydrogenase (G6PDH), was expected to impair NADPH regeneration, thereby limiting glutathione recycling

Tissue sections were incubated with primary mouse antibodies FITC anti-CD192 (1:100 dilution) (CCR2) (BioLegend, 150607), PE anti-CD11b (1:200 dilution) (BioLegend, 101208), anti-CD68 (1:200 dilution) (BioLegend, 137001), anti-F4/80 (1:200 dilution) (BioLegend, 123101), and anti-IL-6 (D5W4V) (1:200 dilution) (Cell Signaling 12912S) overnight, stained with secondary antibody (for CD68, F4/80, IL-6) (Invitrogen SA5-10018, A21434 and A11036)

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