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Once thawed: Centrifuge briefly (30 seconds at 10,000 rpm) to collect any condensation from the tube walls Prepare fresh dilutions in the desired experimental buffer (PBS, cell culture media, etc.) Maintain on ice during experiment setup Use within 24 hours of dilution

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Withdrawal increased anxiety-like behaviors, possibly linked to the upregulation of the GLP-1 pathway

ICH Q2(R2) lists identity, purity, impurity, assay, and qualitative or quantitative measurements as common analytical procedure uses [21]

We examined germline LAG-1::HA accumulation by anti-HA antibody staining in dissected hermaphrodite gonad preparations ( Fig 2B and 2C