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For total protein determination, tissue lysates were solubilized in Laemmli buffer (BioRad, Hercules, CA, USA) and separated by SDS-PAGE using precast TrisHCl gels (BioRad, Hercules, CA, USA)

10.2337/diab.24.8.705 [DOI] [PubMed] [Google Scholar] Falch B

Note: Results may vary based on skin type, lifestyle, and usage consistency

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