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Instrumentation & chromatographic conditions The reverse phase high-performance liquid chromatographic (RP-HPLC) method development and complete partial validation studies was performed with a Waters alliance 2695 Separations Module, comprised of a quaternary pump solvent delivery module, online degasser, thermostated, column compartment, Waters external column heater, auto sampler, auto injector (Model Code SM4) with 100 l injection loop, and a diode-array detector (DAD 2487)

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