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This was followed by transduction with iCas9 and guide expressing vectors (Control and sg Gstt1 ), harvesting by trypsinization, washing twice in PBS, resuspension at 1 x 10 4 in 90ul PBS and 10% Matrigel (Corning), and injected into the 4 th inguinal mammary fat pad of BALB/c mice (Jackson Laboratory) to establish mammary tumors

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