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LC-MS/MS conditions The analyte molecules were eluted on Zorbax XDB-C18 column (3.5 m, 2.1x20 mm) with the mobile phase composed of water/0.1% formic acid and methanol/0.1% formic acid in the ratio of 70:30 V/V with the flow rate of 0.2 ml/min

The technological platforms for studying the microbiome (sets of genes), the metagenome and metabolome, along with high-throughput culture (culturomics) are also presented

Preclinical evidence shows the significance of microbial metabolites and immune signaling in driving these effects, and recent clinical studies further supports these findings (Touchefeu et al., 2014

Evidence based medicine - new approaches and challenges