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The culture medium consisted of 10% fetal bovine serum, 1% penicillin/streptomycin, 2% Ultroser G (Pall Corp.), and 1% ITS Plus (Zen-Bio) to support optimal cell growth

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Migrated cells were fixed with 4% paraformaldehyde and stained with crystal violet

Factors that contribute to these variations include an individual's baseline glutathione levels, skin type, lifestyle, the specific skin concerns being addressed, and the consistency of treatments