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This introduces variability in pharmacodynamics, as GLP-1RA penetration into specific brain regions may differ based on sex, compound properties, and age ( This raises important questions for ongoing trials such as EVOKE, which may uncover sex-specific differences and justify subsequent investigations of GLP-1 analogues in female populations

Future research should prioritize the development of next-generation agonists, explore combination therapies, and integrate precision medicine approaches to optimize patient outcomes

The expression was induced by adding 1 mM IPTG when OD600 reached 0.7-1.2