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Limitations of Anecdotal Evidence Community experience has fundamental limitations that deserve acknowledgment

The cells were first collected by centrifugation at 12,000 g for 5 min, resuspended in RNA later Solution (Ambion), and harvested from the RNA later suspension by centrifugation after overnight storage at 4C

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Increased blood endotoxin levels have been detected in patients with MASLD on comparison with healthy liver controls which also suggests that these could be used an indicative biomarker for progression of liver disease (108)

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