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The observed activity level was consistent with the predicted value of 500 U L 1, confirming the reliability and validity of the RSM analysis under the specified experimental conditions

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Substances are often placed in Category 2 due to: Insufficient human safety data Concerns about impurities or manufacturing variability Lack of clinical justification for traditional pharmacy compounding When a substance is in Category 2, traditional 503A compounding pharmacies generally should not compound it

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