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Urinary sulfate excretion was reduced up to 95% by feeding low-methionine diets to rats, and a 60% decrease in liver methionine was observed, [14]

It is thus notable that H 2 S both inhibits and contributes to respiratory chain activity, suggesting that it induces alternative patterns of electron flow in the respiratory chain 102

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Luciferin detection reagent (LDR) (100 l) was added to all wells, briefly shaken and incubated for 15 min at room temperature and the luminescence measured using microplate imager (BioTek Cytation3)