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The Melanocortin System in Inflammatory Bowel Diseases: Insights Into Its Mechanisms and Therapeutic Potentials

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Briefly, 50 mg of tissue samples was weighted into microwave vessels and 830 L ultrapure water, 900 L 69% HNO 3 ( v/v ) (Suprapure , Merck/Millipore), 250 L 30% H 2 O 2 ( v/v ) (Sigma-Aldrich/Merck), and 20 L of 200 mg/L yttrium-solution (Merck/Millipore) as digestion control were added