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This was to see how GcvA initiates transcription of the glycine biosensor by binding to the gcvBp promoter, how GcvR inhibits GcvA binding to the promoter and how glycine stops GcvR binding to GcvA

Reducing conditions (trace metals, UV light, repeated oxygen exposure from freeze-thaw cycling) can disrupt these disulfide bonds, unfolding the peptide

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This step removes bacteria that have settled on the stoppers during storage and shipping