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Techniques such as liquid chromatographymass spectrometry (LC-MS), direct infusion mass spectrometry, proximity extension assays (Olink), and aptamer-based assays (SomaScan) enable detection of multidimensional biomarker patterns linked to atherosclerosis progression ( Key findings include identification of lipid species such as ceramides, sphingolipids, and glycerolipids that strongly correlate with atherosclerosis progression and cardiovascular events ( Importantly, these platforms provide a scalable approach to evaluate vascular molecular responses to therapies