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Transblotting of the proteins was performed on polyvinylidene difluoride membranes (Merck Millipore) blocked in Tris-buffered saline with 5% skim milk and 0.1% Tween 20 for 1 h and incubated overnight with primary antibodies against IL-1 (1:2,000

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Microscopically, the group treated with boswellic acid indicated an intact cellular framework along with no apparent signs of inflammation, showing the potent anti-inflammatory activity of boswellic acid

In the initial 3 weeks, the observed mean pre-breakfast SMBG levels increased by 0.31 mmol/l from baseline in the IcoSema group and by 0.47 mmol/l in the semaglutide group but had returned to the baseline level by approximately week 6 in both treatment groups