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Cells were washed with PBS and imaged in 11 mM glucose KRBH buffer using a Nikon Eclipse Ti microscope with an ORCA-Flash 4.0 camera (Hamamatsu) and Metamorph software (Molecular Devices) with a 20 air objective and 488 nm laser, with images taken every 6 seconds, on a 37 C stage

Furthermore, the demonstration of cardiovascular benefits across a broad range of adiposity levels, coupled with independence from weight loss magnitude, suggests that semaglutide and perhaps other GLP-1 receptor agonists should be reconceptualized as cardiovascular disease-modifying agents rather than solely medications for glycemic control or weight loss

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Likewise, it is critical to promote awareness of addiction, including AUD, as a chronic relapsing and treatable brain disease [320,321,322]