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HLCs were cultured with Alexa-Flour 488-ac-LDL for 1 hour, followed by immunohistochemistry and DAPI counterstaining [1]

For the D14 dataset, several clusters exhibiting high expression of neutrophil markers, including but not limited to Ly6g , were excluded from the analysis

Lilly is also constructing new manufacturing facilities as part of a USD 27 billion investment drive, with at least three confirmed as production hubs for weight loss therapies [3]

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Follow-Up and Monitoring We schedule follow-up appointments to track your body composition changes, assess progress, and adjust your protocol as needed