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Reconstituted BPC-157 (stored at 2-8 C): 4-6 weeks , ideally less

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Duration: Six to eight weeks, starting as soon after injury as possible

Following this, the receptors were then embedded in a rectangular 126 x 126 1-palmitoyl-2-oleyl-sn-glycerol-3-phosphocholine (POPC) bilayer (previously built by using the VMD Membrane Builder plugin 1.1 at considering the coordinates retrieved from the OPM database to gain the correct orientation within the membrane, while removing the lipid molecules overlapping the receptor TMD bundle