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A 26-week diabetes study measured human activity at 1 mg, but its endpoint was blood sugar rather than long-term weight. A short outpatient cohort measured about 4.7% weight loss over four weeks at 2.5 mg. Below 1 mg, estimates rely on pharmacokinetics and mechanism

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The K m value for CDNB was obtained by reacting 0.5 g of purified e-GSTs of mammals with variable amounts of CDNB (from 0.05 to 2 mM) in the presence of 1 mM GSH, in 0.1 M potassium phosphate buffer, pH 6.5 (25 C)

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The method of sacrifice used for the mice was cervical dislocation, which is a widely recognized and humane method of euthanasia for small rodents such as mice

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tarda were treated with 40 mM NAC, the survival rate was 91% (Figure S2a)

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