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Standard errors for the I ox /I red values were calculated by propagating the signal-to-noise ratio of the individual spectra using the equation below 68 : where I ox /I red is the calculated error in the I ox /I red ratio, I ox is the noise level of the oxidized spectrum, and I red is the noise level of the reduced spectrum

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Notably, dynamic transitions between PCD forms may be regulated by molecules such as p53, Beclin-1, and GPX4

Briefly, Escherichia coli BL21 (DE3) cells (Novagen, Madison, WI, USA) transformed with the pET22b-Ts24GST construct, and were grown in 500 mL of LB medium supplemented with penicillin (100 g/mL)