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What is well-established: Kisspeptin is essential for normal reproductive hormone signaling Kisspeptin-10 and kisspeptin-54 can trigger LH release in clinical settings Kisspeptin-based ovulation triggering in IVF shows genuine clinical promise Kisspeptin dysregulation is measurably involved in PMOS and FHA pathophysiology What is still under investigation: Optimal dosing, timing, and delivery protocols outside IVF contexts Long-term safety data for ongoing or cyclical therapeutic use Clinical protocols for kisspeptin in anovulation management outside of assisted reproduction Whether exogenous kisspeptin-10 (the shorter fragment) produces equivalent clinical effects to kisspeptin-54 in fertility contexts As with many emerging peptide therapies, kisspeptin should be approached with informed expectations

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To do so, stable isotope labeling and mass spectrometry imaging are leveraged via infrared matrix-assisted laser desorption electrospray ionization (IR-MALDESI)

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